The QIAcuity Digital PCR System makes absolute quantification accessible and affordable for all laboratories. The walk-away automation integrates and streamlines the entire digital PCR workflow of partitioning, thermocycling and imaging into a single instrument with minimal hands-on time. It is also easy to adapt your current qPCR assays to the QIAcuity Digital PCR System. No change in plate handling is required when coming from qPCR, assuring fast assay setup and quick results in under 2 hours.
QIAcuity Instruments – features and specifications
|Feature||QIAcuity One||QIAcuity Four||QIAcuity Eight|
|Detection channels (multiplexing)||2 or 5||5||5|
|Time to result||Under 2 h||First plate under 2 h
Every ~60 min a following plate
|First plate under 2 h
Every ~30 min a following plate
|Throughput (samples processed in a work day)||Up to 384 (96-well)
Up to 96 (24-well)
|Up to 672 (96-well)
Up to 168 (24-well)
|Up to 1248 (96-well)
Up to 312 (24-well)
In just 3 simple steps, you can have the dPCR result you want in under 2 hours: pipette and load, run the experiment, analyze results.
The principle of the dPCR reaction in the nanoplates is described here.
Just like in qPCR experiments, sample preparation includes the transfer of master mix, probes and primers to a 96- or 24-well nanoplate, followed by the addition of samples. The system integrates partitioning, thermocycling and imaging into a single fully automated instrument that takes users from the sample to result in under 2 hours. One can perform analysis on the Software Suite, providing the concentration in copies per microliter of your target sequence as well as for quality control such as positive samples or NTC. This analysis can also be extended to remote computers within the same local area network (LAN).
The QIAcuity instruments, together with the QIAcuity Nanoplates and the QIAcuity PCR kits, enable digital PCR applications, including:
- Rare mutation detection
- Copy number variation analysis
- Gene expression analysis
- Pathogen detection
- miRNA research
The QIAcuity Software Suite provided with the instrument and installed on a separate computer controls one or multiple QIAcuity instruments, either connected directly to one instrument or using an existing local area network (LAN). Using the QIAcuity Software Suite, digital PCR experiments, samples and reaction mixes can be defined, assigned to Nanoplates, and transferred to the QIAcuity instrument. After the run, data can be analyzed, reports can be created and data can be exported for external analysis. The software provides multiple template functionalities to make repeating plate layouts or plate run parameters easily accessible, further transforming your digital PCR experience.
When integrated into a local area network, the computer is hosting the QIAcuity Software Suite function as a server that is accessible via LAN to other computers serving as clients. This enables multiple users to access the software from other rooms or offices and analyze data via a standard browser without the need to install the software on multiple computers or access and exchange data via internet connections.