The QuantiNova Pathogen +IC Kit is designed for sensitive, rapid real-time (RT-) PCR detection of pathogen nucleic acids using sequence-specific probes. The kit contains reagents for 4-plex real-time detection of user-defined pathogen targets (e.g., virus, bacteria, fungi, etc.) plus the QuantiNova Internal Control (IC) RNA or DNA. Both ICs and assay are included for use as extraction or amplification controls, allowing correct interpretation of negative detection results. Furthermore, the ultrafast, ready-to-use kit offers convenient room-temperature reaction setup for a streamlined, automated workflow, as well as a visual pipetting control to increase handling and safety standards. For convenience, the master mix can be stored at 2–8°C.